Chien Ho

[1987a] Ho C, IM Russu How much do we know about the Bohr effect of hemoglobin? Biochemistry 26: 6299-6305

[1988a] Peng ZY, V Simplaceanu, IJ Lowe, C Ho Rotating-frame relaxation studies of slow motions in fluorinated phospholipid model membranes. Biophys J 54: 81-95

[1989a] Buckel SD, PF Cottam, V Simplaceanu, C Ho Formation of intermolecular and intramolecular hydrogen bonds in histidine-binding protein J of Salmonella typhimurium upon binding L-histidine. A proton nuclear magnetic resonance study. J Mol Biol 208: 477-489

[1989b] Ho C, EA Pratt, GS Rule Membrane-bound D-lactate dehydrogenase of Escherichia coli: a model for protein interactions in membranes. Biochim Biophys Acta 988: 173-184

[1989c] Peng ZY, V Simplaceanu, SR Dowd, C Ho Effects of cholesterol or gramicidin on slow and fast motions of phospholipids in oriented bilayers. Proc Natl Acad Sci U S A 86: 8758-8762

[1989d] Peng ZY, N Tjandra, V Simplaceanu, C Ho Slow motions in oriented phospholipid bilayers and effects of cholesterol or gramicidin. A 19F-NMR T1 rho study. Biophys J 56: 877-885

[1989e] Russu IM, SS Wu, NT Ho, GW Kellogg, C Ho A proton nuclear magnetic resonance investigation of the anion Bohr effect of human normal adult hemoglobin. Biochemistry 28: 5298-5306

[1989f] Shen QC, V Simplaceanu, PF Cottam, C Ho Proton nuclear magnetic resonance studies on glutamine-binding protein from Escherichia coli. Formation of intermolecular and intramolecular hydrogen bonds upon ligand binding. J Mol Biol 210: 849-857

[1989g] Shen QC, V Simplaceanu, PF Cottam, JL Wu, JS Hong, C Ho Molecular genetic, biochemical and nuclear magnetic resonance studies on the role of the tryptophan residues of glutamine-binding protein from Escherichia coli. J Mol Biol 210: 859-867

[1990a] Busch MR, CE Ho Effects of anions on the molecular basis of the Bohr effect of hemoglobin. Biophys Chem 37: 313-322

[1990b] Detre JA, AP Koretsky, DS Williams, C Ho Absence of pH changes during altered work in the in vivo sheep heart: a 31P-NMR investigation. J Mol Cell Cardiol 22: 543-553

[1990c] Groebe DR, AE Chung, C Ho Cationic lipid-mediated co-transfection of insect cells. Nucleic Acids Res 18: 4033

[1990d] Peersen OB, EA Pratt, HT Truong, C Ho, GS Rule Site-specific incorporation of 5-fluorotryptophan as a probe of the structure and function of the membrane-bound D-lactate dehydrogenase of Escherichia coli: a 19F nuclear magnetic resonance study. Biochemistry 29: 3256-3262

[1990e] Russu IM, SS Wu, KA Bupp, NT Ho, C Ho 1H and 31P nuclear magnetic resonance investigation of the interaction between 2,3-diphosphoglycerate and human normal adult hemoglobin. Biochemistry 29: 3785-3792

[1991a] Busch MR, JE Mace, NT Ho, C Ho Roles of the beta 146 histidyl residue in the molecular basis of the Bohr effect of hemoglobin: a proton nuclear magnetic resonance study. Biochemistry 30: 1865-1877

[1991b] Truong HT, EA Pratt, C Ho Interaction of the membrane-bound D-lactate dehydrogenase of Escherichia coli with phospholipid vesicles and reconstitution of activity using a spin-labeled fatty acid as an electron acceptor: a magnetic resonance and biochemical study. Biochemistry 30: 3893-3898

[1991c] Truong HT, EA Pratt, GS Rule, PY Hsue, C Ho Inactive and temperature-sensitive folding mutants generated by tryptophan substitutions in the membrane-bound d-lactate dehydrogenase of Escherichia coli. Biochemistry 30: 10722-10729

[1992a] Groebe DR, MR Busch, TY Tsao, FY Luh, MF Tam, AE Chung, M Gaskell, SA Liebhaber, C Ho High-level production of human alpha- and beta-globins in insect cells. Protein Expr Purif 3: 134-141

[1992b] Ho C Proton nuclear magnetic resonance studies on hemoglobin: cooperative interactions and partially ligated intermediates. Adv Protein Chem 43: 153-312

[1992c] Tjandra N, V Simplaceanu, PF Cottam, C Ho Multidimensional 1H and 15N NMR investigation of glutamine-binding protein of Escherichia coli. J Biomol NMR 2: 149-160

[1993a] Schrader MC, CJ Eskey, V Simplaceanu, C Ho A carbon-13 nuclear magnetic resonance investigation of the metabolic fluxes associated with glucose metabolism in human erythrocytes. Biochim Biophys Acta 1182: 162-178

[1993b] Schrader MC, V Simplaceanu, C Ho Measurement of fluxes through the pentose phosphate pathway in erythrocytes from individuals with sickle cell anemia by carbon-13 nuclear magnetic resonance spectroscopy. Biochim Biophys Acta 1182: 179-188

[1993c] Shen TJ, NT Ho, V Simplaceanu, M Zou, BN Green, MF Tam, C Ho Production of unmodified human adult hemoglobin in Escherichia coli. Proc Natl Acad Sci U S A 90: 8108-8112

[1993d] Sun ZY, HT Truong, EA Pratt, DC Sutherland, CE Kulig, RJ Homer, SM Groetsch, PY Hsue, C Ho A 19F-NMR study of the membrane-binding region of D-lactate dehydrogenase of Escherichia coli. Protein Sci 2: 1938-1947

[1993e] Yeh TC, W Zhang, ST Ildstad, C Ho Intracellular labeling of T-cells with superparamagnetic contrast agents. Magn Reson Med 30: 617-625

[1994a] Ho C, JR Perussi Proton nuclear magnetic resonance studies of hemoglobin. Methods Enzymol 232: 97-139

[1994b] Kim HW, TJ Shen, DP Sun, NT Ho, M Madrid, MF Tam, M Zou, PF Cottam, C Ho Restoring allosterism with compensatory mutations in hemoglobin. Proc Natl Acad Sci U S A 91: 11547-11551

[1994c] Zhang W, C Ho Lactate mapping with full sensitivity by spin-filtered NMR imaging. J Magn Reson B 103: 120-127

[1995a] Dowd SR, EA Pratt, ZY Sun, C Ho Nature and environment of the sulfhydryls of membrane-associated D-lactate dehydrogenase of Escherichia coli. Biochim Biophys Acta 1252: 278-283

[1995b] Fetler BK, V Simplaceanu, C Ho 1H-NMR investigation of the oxygenation of hemoglobin in intact human red blood cells. Biophys J 68: 681-693

[1995c] Kim HW, TJ Shen, DP Sun, NT Ho, M Madrid, C Ho A novel low oxygen affinity recombinant hemoglobin (alpha96val--> Trp): switching quaternary structure without changing the ligation state. J Mol Biol 248: 867-882

[1995d] Sun ZY, SR Dowd, C Felix, JS Hyde, C Ho Stopped-flow kinetic and biophysical studies of membrane-associated D-lactate dehydrogenase of Escherichia coli. Biochim Biophys Acta 1252: 269-277

[1995e] Yeh TC, W Zhang, ST Ildstad, C Ho In vivo dynamic MRI tracking of rat T-cells labeled with superparamagnetic iron-oxide particles. Magn Reson Med 33: 200-208

[1996a] Ho C, BF Willis, TJ Shen, NT Dazhen, DP Sun, MF Tam, SM Suzuka, ME Fabry, RL Nagel Roles of alpha 114 and beta 87 amino acid residues in the polymerization of hemoglobin S: implications for gene therapy. J Mol Biol 263: 475-485

[1996b] Kim HW, TJ Shen, NT Ho, M Zou, MF Tam, C Ho Contributions of asparagine at alpha 97 to the cooperative oxygenation process of hemoglobin. Biochemistry 35: 6620-6627

[1996c] Sun ZY, EA Pratt, V Simplaceanu, C Ho A 19F-NMR study of the equilibrium unfolding of membrane-associated D-lactate dehydrogenase of Escherichia coli. Biochemistry 35: 16502-16509

[1997a] Lukin JA, AP Gove, SN Talukdar, C Ho Automated probabilistic method for assigning backbone resonances of (13C,15N)-labeled proteins. J Biomol NMR 9: 151-166

[1997b] Shen TJ, NT Ho, M Zou, DP Sun, PF Cottam, V Simplaceanu, MF Tam, DA Bell, Jr., C Ho Production of human normal adult and fetal hemoglobins in Escherichia coli. Protein Eng 10: 1085-1097

[1997c] Sun DP, M Zou, NT Ho, C Ho Contribution of surface histidyl residues in the alpha-chain to the Bohr effect of human normal adult hemoglobin: roles of global electrostatic effects. Biochemistry 36: 6663-6673

[1997d] Yu J, V Simplaceanu, NL Tjandra, PF Cottam, JA Lukin, C Ho 1H, 13C, and 15N NMR backbone assignments and chemical-shift-derived secondary structure of glutamine-binding protein of Escherichia coli. J Biomol NMR 9: 167-180

[1998a] Wang JJ, KS Hendrich, EK Jackson, ST Ildstad, DS Williams, C Ho Perfusion quantitation in transplanted rat kidney by MRI with arterial spin labeling. Kidney Int 53: 1783-1791

[1998b] Yoshida S, K Takeuchi, PJ del Nido, C Ho Diastolic dysfunction coincides with early mild transplant rejection: in situ measurements in a heterotopic rat heart transplant model. J Heart Lung Transplant 17: 1049-1056

[1999a] Dodd SJ, M Williams, JP Suhan, DS Williams, AP Koretsky, C Ho Detection of single mammalian cells by high-resolution magnetic resonance imaging. Biophys J 76: 103-109

[1999b] Fang TY, M Zou, V Simplaceanu, NT Ho, C Ho Assessment of roles of surface histidyl residues in the molecular basis of the Bohr effect and of beta 143 histidine in the binding of 2,3-bisphosphoglycerate in human normal adult hemoglobin. Biochemistry 38: 13423-13432

[1999c] Hendrich KS, PM Kochanek, DS Williams, JK Schiding, DW Marion, C Ho Early perfusion after controlled cortical impact in rats: quantification by arterial spin-labeled MRI and the influence of spin-lattice relaxation time heterogeneity. Magn Reson Med 42: 673-681

[1999d] Jeong ST, NT Ho, MP Hendrich, C Ho Recombinant hemoglobin(alpha 29leucine --> phenylalanine, alpha 96valine --> tryptophan, beta 108asparagine --> lysine) exhibits low oxygen affinity and high cooperativity combined with resistance to autoxidation. Biochemistry 38: 13433-13442

[1999e] Larson SC, GW Fisher, NT Ho, TJ Shen, C Ho A biochemical and biophysical characterization of recombinant mutants of fetal hemoglobin and their interaction with sickle cell hemoglobin. Biochemistry 38: 9549-9555

[1999f] Shen TJ, P Ikonomi, R Smith, CT Noguchi, C Ho Multi-ribozyme targeting of human alpha-globin gene expression. Blood Cells Mol Dis 25: 361-373

[1999g] Tsai CH, TJ Shen, NT Ho, C Ho Effects of substitutions of lysine and aspartic acid for asparagine at beta 108 and of tryptophan for valine at alpha 96 on the structural and functional properties of human normal adult hemoglobin: roles of alpha 1 beta 1 and alpha 1 beta 2 subunit interfaces in the cooperative oxygenation process. Biochemistry 38: 8751-8761

[1999h] Yoshida S, SJ Dodd, PJ del Nido, DS Williams, C Ho Cardiac function of transplanted rat hearts using a working heart model assessed by magnetic resonance imaging. J Heart Lung Transplant 18: 1054-1064

[2000a] Fang TY, V Simplaceanu, CH Tsai, NT Ho, C Ho An additional H-bond in the alpha 1 beta 2 interface as the structural basis for the low oxygen affinity and high cooperativity of a novel recombinant hemoglobin (beta L105W). Biochemistry 39: 13708-13718

[2000b] Kanno S, PC Lee, SJ Dodd, M Williams, BP Griffith, C Ho A novel approach with magnetic resonance imaging used for the detection of lung allograft rejection. J Thorac Cardiovasc Surg 120: 923-934

[2000c] Kanno S, PC Lee, Y Zhang, C Ho, BP Griffith, LL Shears, 2nd, TR Billiar Attenuation of myocardial ischemia/reperfusion injury by superinduction of inducible nitric oxide synthase. Circulation 101: 2742-2748

[2000d] Lukin JA, V Simplaceanu, M Zou, NT Ho, C Ho NMR reveals hydrogen bonds between oxygen and distal histidines in oxyhemoglobin. Proc Natl Acad Sci U S A 97: 10354-10358

[2000e] Simplaceanu V, JA Lukin, TY Fang, M Zou, NT Ho, C Ho Chain-selective isotopic labeling for NMR studies of large multimeric proteins: application to hemoglobin. Biophys J 79: 1146-1154

[2000f] Tsai CH, TY Fang, NT Ho, C Ho Novel recombinant hemoglobin, rHb (beta N108Q), with low oxygen affinity, high cooperativity, and stability against autoxidation. Biochemistry 39: 13719-13729

[2000g] Zhang Y, SJ Dodd, KS Hendrich, M Williams, C Ho Magnetic resonance imaging detection of rat renal transplant rejection by monitoring macrophage infiltration. Kidney Int 58: 1300-1310

[2001a] Hendrich KS, PM Kochanek, JA Melick, JK Schiding, KD Statler, DS Williams, DW Marion, C Ho Cerebral perfusion during anesthesia with fentanyl, isoflurane, or pentobarbital in normal rats studied by arterial spin-labeled MRI. Magn Reson Med 46: 202-206

[2001b] Kanno S, YJ Wu, PC Lee, TR Billiar, C Ho Angiotensin-converting enzyme inhibitor preserves p21 and endothelial nitric oxide synthase expression in monocrotaline-induced pulmonary arterial hypertension in rats. Circulation 104: 945-950

[2001c] Kanno S, YJ Wu, PC Lee, SJ Dodd, M Williams, BP Griffith, C Ho Macrophage accumulation associated with rat cardiac allograft rejection detected by magnetic resonance imaging with ultrasmall superparamagnetic iron oxide particles. Circulation 104: 934-938

[2001d] Tsai CH, SC Larson, TJ Shen, NT Ho, GW Fisher, MF Tam, C Ho Probing the importance of the amino-terminal sequence of the beta- and gamma-chains to the properties of normal adult and fetal hemoglobins. Biochemistry 40: 12169-12177

[2001e] Yang D, Q Ye, M Williams, Y Sun, TC Hu, DS Williams, JM Moura, C Ho USPIO-enhanced dynamic MRI: evaluation of normal and transplanted rat kidneys. Magn Reson Med 46: 1152-1163

[2002a] Chang CK, V Simplaceanu, C Ho Effects of amino acid substitutions at beta 131 on the structure and properties of hemoglobin: evidence for communication between alpha 1 beta 1- and alpha 1 beta 2-subunit interfaces. Biochemistry 41: 5644-5655

[2002b] Cheng Y, TJ Shen, V Simplaceanu, C Ho Ligand binding properties and structural studies of recombinant and chemically modified hemoglobins altered at beta 93 cysteine. Biochemistry 41: 11901-11913

[2002c] Dodd SJ, C Ho Short planar gradient coils for MR microscopy using concentric return paths. J Magn Reson 156: 1-9

[2002d] Huang MQ, Q Ye, DS Williams, C Ho MRI of lungs using partial liquid ventilation with water-in-perfluorocarbon emulsions. Magn Reson Med 48: 487-492

[2002e] Tsai CH, C Ho Recombinant hemoglobins with low oxygen affinity and high cooperativity. Biophys Chem 98: 15-25

[2002f] Ye Q, D Yang, M Williams, DS Williams, C Pluempitiwiriyawej, JM Moura, C Ho In vivo detection of acute rat renal allograft rejection by MRI with USPIO particles. Kidney Int 61: 1124-1135

[2002g] Yuan Y, V Simplaceanu, JA Lukin, C Ho NMR investigation of the dynamics of tryptophan side-chains in hemoglobins. J Mol Biol 321: 863-878

[2003a] Lukin JA, C Ho Nuclear magnetic resonance of hemoglobins. Methods Mol Med 82: 251-269

[2003b] Lukin JA, G Kontaxis, V Simplaceanu, Y Yuan, A Bax, C Ho Quaternary structure of hemoglobin in solution. Proc Natl Acad Sci U S A 100: 517-520

[2003c] Sun Y, D Yang, Q Ye, M Williams, JM Moura, F Boada, ZP Liang, C Ho Improving spatiotemporal resolution of USPIO-enhanced dynamic imaging of rat kidneys. Magn Reson Imaging 21: 593-598

[2003d] Tsai CH, V Simplaceanu, NT Ho, TJ Shen, D Wang, TG Spiro, C Ho Site mutations disrupt inter-helical H-bonds (alpha14W-alpha67T and beta15W-beta72S) involved in kinetic steps in the hemoglobin R-->T transition without altering the free energies of oxygenation. Biophys Chem 100: 131-142

[2004a] Ho C, TK Hitchens A non-invasive approach to detecting organ rejection by MRI: monitoring the accumulation of immune cells at the transplanted organ. Curr Pharm Biotechnol 5: 551-566

[2004b] Huang MQ, PH Basse, Q Yang, JA Horner, TK Hichens, C Ho MRI detection of tumor in mouse lung using partial liquid ventilation with a perfluorocarbon-in-water emulsion. Magn Reson Imaging 22: 645-652

[2004c] Lukin JA, C Ho The structure--function relationship of hemoglobin in solution at atomic resolution. Chem Rev 104: 1219-1230

[2004d] Wu YJ, K Sato, Q Ye, C Ho MRI investigations of graft rejection following organ transplantation using rodent models. Methods Enzymol 386: 73-105

[2004e] Yang D, Q Ye, DS Williams, TK Hitchens, C Ho Normal and transplanted rat kidneys: diffusion MR imaging at 7 T. Radiology 231: 702-709

[2005a] Foley LM, TK Hitchens, PM Kochanek, JA Melick, EK Jackson, C Ho Murine orthostatic response during prolonged vertical studies: effect on cerebral blood flow measured by arterial spin-labeled MRI. Magn Reson Med 54: 798-806

[2005b] Wiltrout ME, JL Giovannelli, V Simplaceanu, JA Lukin, NT Ho, C Ho A biophysical investigation of recombinant hemoglobins with aromatic B10 mutations in the distal heme pockets. Biochemistry 44: 7207-7217

[2006a] Chang HH, JM Moura, YL Wu, C Ho Immune cells detection of the in vivo rejecting heart in USPIO-enhanced magnetic resonance imaging. Conf Proc IEEE Eng Med Biol Soc 1: 942-945

[2006b] Gong Q, V Simplaceanu, JA Lukin, JL Giovannelli, NT Ho, C Ho Quaternary structure of carbonmonoxyhemoglobins in solution: structural changes induced by the allosteric effector inositol hexaphosphate. Biochemistry 45: 5140-5148

[2006c] Sahu SC, V Simplaceanu, Q Gong, NT Ho, JG Glushka, JH Prestegard, C Ho Orientation of deoxyhemoglobin at high magnetic fields: structural insights from RDCs in solution. J Am Chem Soc 128: 6290-6291

[2006d] Sahu SC, V Simplaceanu, NT Ho, JL Giovannelli, C Ho Backbone resonance assignment of human adult hemoglobin in the deoxy form. J Biomol NMR 36 Suppl 1: 1

[2006e] Wu YL, Q Ye, LM Foley, TK Hitchens, K Sato, JB Williams, C Ho In situ labeling of immune cells with iron oxide particles: an approach to detect organ rejection by cellular MRI. Proc Natl Acad Sci U S A 103: 1852-1857

[2007a] Sahu SC, V Simplaceanu, Q Gong, NT Ho, F Tian, JH Prestegard, C Ho Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector. Biochemistry 46: 9973-9980

[2007b] Shen TJ, H Rogers, X Yu, F Lin, CT Noguchi, C Ho Modification of globin gene expression by RNA targeting strategies. Exp Hematol 35: 1209-1218

[2007c] Song XJ, Y Yuan, V Simplaceanu, SC Sahu, NT Ho, C Ho A comparative NMR study of the polypeptide backbone dynamics of hemoglobin in the deoxy and carbonmonoxy forms. Biochemistry 46: 6795-6803

[2007d] Williams JB, Q Ye, TK Hitchens, CL Kaufman, C Ho MRI detection of macrophages labeled using micrometer-sized iron oxide particles. J Magn Reson Imaging 25: 1210-1218

[2008a] Foley LM, TK Hitchens, JA Melick, H Bayir, C Ho, PM Kochanek Effect of inducible nitric oxide synthase on cerebral blood flow after experimental traumatic brain injury in mice. J Neurotrauma 25: 299-310

[2008b] Maillett DH, V Simplaceanu, TJ Shen, NT Ho, JS Olson, C Ho Interfacial and distal-heme pocket mutations exhibit additive effects on the structure and function of hemoglobin. Biochemistry 47: 10551-10563

[2008c] Mills PH, YJ Wu, C Ho, ET Ahrens Sensitive and automated detection of iron-oxide-labeled cells using phase image cross-correlation analysis. Magn Reson Imaging 26: 618-628

[2008d] Song XJ, V Simplaceanu, NT Ho, C Ho Effector-induced structural fluctuation regulates the ligand affinity of an allosteric protein: binding of inositol hexaphosphate has distinct dynamic consequences for the T and R states of hemoglobin. Biochemistry 47: 4907-4915

[2008e] Ye Q, YL Wu, LM Foley, TK Hitchens, DF Eytan, H Shirwan, C Ho Longitudinal tracking of recipient macrophages in a rat chronic cardiac allograft rejection model with noninvasive magnetic resonance imaging using micrometer-sized paramagnetic iron oxide particles. Circulation 118: 149-156